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cellular senescence detection kit spider β gal  (Dojindo Labs)


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    Dojindo Labs cellular senescence detection kit spider β gal
    Cellular Senescence Detection Kit Spider β Gal, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellular+senescence+detection+kit+spider+%CE%B2+gal/Cellular+Senescence+Detection+Kit+-+SPiDER-BGal/pmc12905430-78-1-5
    Average 95 stars, based on 88 article reviews
    cellular senescence detection kit spider β gal - by Bioz Stars, 2026-09
    95/100 stars

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    Cell Culture:

    Article Title: Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N -Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin
    Article Snippet: After fixation, the cells were incubated with anti-CD73 (1 : 50; Proteintech Group, Inc., Rosemont, IL, USA) or anti-CD105 (1 : 50; Bioss Antibodies Inc., Woburn, MA, USA) at 4°C for 1 h and then incubated with CF488-conjugated antirabbit IgG secondary antibody (1 : 500; Biotium) at 4°C for 1 h. Mouse IgG (Wako Pure Chemical Industries, Ltd., Osaka, Japan) and rabbit IgG (Thermo Fisher Scientific Inc.) were used as isotype controls. .. To assess for cellular senescence of MSCs, the harvested cells were cultured with 10% FBS/DMEM for 1 day and stained using the Cellular Senescence Detection Kit-SPiDER- β Gal (Dojindo). .. Stained cells were then analyzed using a Guava EasyCyte Flow Cytometry System (EMD Millipore).

    Staining:

    Article Title: Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N -Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin
    Article Snippet: After fixation, the cells were incubated with anti-CD73 (1 : 50; Proteintech Group, Inc., Rosemont, IL, USA) or anti-CD105 (1 : 50; Bioss Antibodies Inc., Woburn, MA, USA) at 4°C for 1 h and then incubated with CF488-conjugated antirabbit IgG secondary antibody (1 : 500; Biotium) at 4°C for 1 h. Mouse IgG (Wako Pure Chemical Industries, Ltd., Osaka, Japan) and rabbit IgG (Thermo Fisher Scientific Inc.) were used as isotype controls. .. To assess for cellular senescence of MSCs, the harvested cells were cultured with 10% FBS/DMEM for 1 day and stained using the Cellular Senescence Detection Kit-SPiDER- β Gal (Dojindo). .. Stained cells were then analyzed using a Guava EasyCyte Flow Cytometry System (EMD Millipore).

    Article Title: Immunometabolic effects of lactate on humoral immunity in healthy individuals of different ages
    Article Snippet: For phosphorylated STAT3 (p-STAT3), CD4+ T cells were membrane stained initially with Live/Dead kit and anti-CD4 (Biolegend 317410), then fixed, permeabilized with PBS-0.2% Tween, then stained for additional 20 min at room temperature with anti-phosho-STAT3 antibody (Tyr 705, Cell Signaling 4113S), followed by an anti-IgG (H + L) secondary conjugated antibody (Thermo Fisher A11029). .. For β-Galactosidase staining, we used the Cellular Senescence detection kit SPiDER β-Gal (Dojindo Molecular Technologies SG04) at concentrations recommended by the manufacturer, but after membrane staining. .. In every experiment we acquired up to 10 5 events in the B cell gate on a LSR-Fortessa (BD).

    Activity Assay:

    Article Title: ACSL1 promotes imatinib-induced chronic myeloid leukemia cell senescence by regulating SIRT1/p53/p21 pathway.
    Article Snippet: .. SA-β-gal activity was detected using a Cellular Senescence Detection Kit-SPiDER-β Gal (Dojindo, Shanghai, China). ..

    Article Title: ACSL1 promotes imatinib-induced chronic myeloid leukemia cell senescence by regulating SIRT1/p53/p21 pathway
    Article Snippet: The results were analyzed on FlowJo V10 (Stanford University, San Francisco, CA, USA). .. SA-β-gal activity was detected using a Cellular Senescence Detection Kit-SPiDER-β Gal (Dojindo, Shanghai, China). ..

    Membrane:

    Article Title: Immunometabolic effects of lactate on humoral immunity in healthy individuals of different ages
    Article Snippet: For phosphorylated STAT3 (p-STAT3), CD4+ T cells were membrane stained initially with Live/Dead kit and anti-CD4 (Biolegend 317410), then fixed, permeabilized with PBS-0.2% Tween, then stained for additional 20 min at room temperature with anti-phosho-STAT3 antibody (Tyr 705, Cell Signaling 4113S), followed by an anti-IgG (H + L) secondary conjugated antibody (Thermo Fisher A11029). .. For β-Galactosidase staining, we used the Cellular Senescence detection kit SPiDER β-Gal (Dojindo Molecular Technologies SG04) at concentrations recommended by the manufacturer, but after membrane staining. .. In every experiment we acquired up to 10 5 events in the B cell gate on a LSR-Fortessa (BD).

    Flow Cytometry:

    Article Title: Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer
    Article Snippet: All Data were analyzed using FlowJo v10.1r5 software (Ashland, OR, USA). .. Cellular senescence was evaluated using a Cellular Senescence Detection Kit SPiDER-β-Gal (Dojindo, Kumamoto, Japan) in conjunction with flow cytometry. ..



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